Melike Lakadamyali: Super-resolution imaging of chromatin structure and dynamics

Mon, Nov 25, 2019, 12:00 pm
Location: 
Joseph Henry Room Jadwin Hall
Speaker(s): 
Sponsor(s): 
CPBF an NSF PFC

Nucleosomes help structure chromosomes by compacting DNA into fibers.  Chromatin organization plays an important role for regulating gene expression; however, due to the highly crowded nuclear environment and the nanometer length scales of chromatin fibers, it has been very difficult to visualize chromatin in vivo. We have overcome this challenge by developing highly quantitative and multiplexed super-resolution microscopy methods that allow us to not only visualize chromatin with nanoscale spatial and kilobase genomic resolution but also allow us to count the number of nucleosomes along the chromatin fiber. Our results reveal a new paradigm of chromatin compaction in the form of heterogeneous groups of nucleosomes, which we termed nucleosome clutches, in analogy to egg clutches. We have further shown that the nanoscale chromatin organization is highly cell-type specific and correlates with the level of cell pluripotency. Using single molecule tracking, we have also uncovered a heterogeneous chromatin mobility landscape that is both cell-type specific and that functionally maps to the mobility of canonical as well as pioneer transcription factors. Overall, our results are revealing new insights into the intimate link between chromatin structure, dynamics and gene activity.

Melike Lakadamyali: Super-resolution imaging of chromatin structure and dynamics

Mon, Nov 25, 2019, 12:00 pm

Nucleosomes help structure chromosomes by compacting DNA into fibers.  Chromatin organization plays an important role for regulating gene...

Location: Joseph Henry Room Jadwin Hall

Damon Clark: Mechanisms underlying visual illusions in flies

Mon, Dec 2, 2019, 12:00 pm

Visual systems detect many features of natural scenes, including motion. Motion detection guides critical behaviors like hunting, evading...

Location: Joseph Henry Room Jadwin Hall

Adaptation

Fri, Dec 6, 2019, 9:30 am

Adaptation and Bayesian forecasting in biological systems
Thierry Mora, École Normale Supérieure and Princeton University...

Location: The Graduate Center, Segal Theater. 365 Fifth Avenue, between 34th and 35th Streets, in Manhattan

Hana El-Samad: Biological Feedback Control

Mon, Dec 9, 2019, 12:00 pm

Organisms are an evolutionary masterpiece of feedback control, featuring a mind-boggling capacity to self-correct.

Location: Joseph Henry Room Jadwin Hall

Navish Wadhwa: TBD

Mon, Dec 16, 2019, 12:00 pm
Location: Joseph Henry Room Jadwin Hall

Dmitry Rinberg: TBD

Mon, Jan 27, 2020, 12:00 pm
Location: Joseph Henry Room Jadwin Hall

Sven van Taeffelen: TBD

Mon, Feb 3, 2020, 12:00 pm
Location: Joseph Henry Room Jadwin Hall

Elizabeth Jerison: TBD

Mon, Feb 10, 2020, 12:00 pm
Location: Joseph Henry Room Jadwin Hall

Elizabeth Jerison: TBD

Mon, Feb 10, 2020, 12:00 pm
Location: Joseph Henry Room Jadwin Hall

Stephanie Palmer: TBD

Mon, Feb 17, 2020, 12:00 pm
Location: Joseph Henry Room Jadwin Hall

Andrew York: TBD

Mon, Feb 24, 2020, 12:00 pm
Location: Joseph Henry Room Jadwin Hall

Andreas Gahlman: TBD

Mon, Mar 9, 2020, 12:00 pm
Location: Joseph Henry Room Jadwin Hall